Composite

Part:BBa_K1992007

Designed by: Shilo Ohayon, Tzila Davidov   Group: iGEM16_Technion_Israel   (2016-10-14)
Revision as of 19:55, 20 October 2016 by Inbalad (Talk | contribs)

PctA-Tar expression system (promoter+RBS+coding+terminator)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 331
    Illegal NgoMIV site found at 412
    Illegal NgoMIV site found at 416
    Illegal NgoMIV site found at 435
    Illegal AgeI site found at 535
    Illegal AgeI site found at 746
    Illegal AgeI site found at 1601
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

Here is the plasmid which serves as the plasmid of the PctA-Tar chimera. The plasmid is comprised of a promoter, a strong RBS , the chimera and a double terminator.

Usage and Biology

Essentially, the expression of the chimera is enabled independently by this device, while taking advantage of the bacterium expression resources. For methods and results please go to PctA-Tar (BBa_K1992001)


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Categories
Parameters
None