Composite

Part:BBa_K1900002

Designed by: Elise Sloey   Group: iGEM16_WLC-Milwaukee   (2016-10-12)
Revision as of 16:59, 20 October 2016 by Elisesloey (Talk | contribs)

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pBAD+strong RBS+E. coli tolC signal sequence+E. coli tolC Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the E. coli tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 265
    Illegal NheI site found at 1615
    Illegal NheI site found at 1636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1110


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