Reporter

Part:BBa_K1980009

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 13:39, 20 October 2016 by AndreasH. (Talk | contribs)


pCusC CusR RFP

pCusC CusR RFP

Description

This synthetic promoter system includes the response regulator CusR (part of the CusR/S copper sensitive two-component system found in E. coli) and the an RFP variant (mKate) expressed downstream of a copper sensitive promoter (pCusC). The fact that CusR is found downstream of the promoter it activates means that a positive feedback loop is created when sufficient copper is sensed in the environment

<img src="T--Oxford--pCusC_CusR_RFP.jpg"/>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/rfp
//function/sensor/metal
//promoter
Parameters
None