Coding
Part:BBa_K1921018
Designed by: Zhuozhi Chen Group: iGEM16_TJUSLS_China (2016-10-14)
LPP-OmpA+linker.a
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
We add a GGGGSGGGGS linker at the C-terminal of Lpp-OmpA in order to minimize its potential influence on our target protein.
Pre-expression
Figure 1.This is the pre-expression using E.coli BL21 at 37 ℃.
Figure 2. This is the pre-expression using E.coli BL21 at 16 ℃.
Figure 3. This is the pre-expression using E.coli BL21 at 25 ℃.
Surface display HPLC results
Figure 4. Relative enzyme activity of engineering bacteria E.coli(BL21)/pET22b(+)LAP when induced at 16℃.
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Categories
Parameters
//awards/part_collection/2016
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