DNA

Part:BBa_K2100004:Experience

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)
Revision as of 01:55, 20 October 2016 by Sarahcaso (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2100004

We characterized our TRE promoter induced with a fine sweep of Doxycycline from a concentration of 0 uM to 2000 uM. We transfected HEK293 cells with 250ng of TRE-mKate and 250 ng hEF1a-eYFP. We analyzed fluorescent output in the PE Texas Red channel to measure the activity of the TRE promoter under different DOX concentrations. We used 0 uM of DOX as a control.

T--MIT--TRE.png

The results indicate the optimal DOX concentration was 500 uM for maximum TRE activity. Beyond that point, adding a higher concentration of DOX does not significantly increase fluorescent output from the TRE-mKate construct.


User Reviews

UNIQdc263a24cd940733-partinfo-00000000-QINU UNIQdc263a24cd940733-partinfo-00000001-QINU