Regulatory

Part:BBa_K124000:Experience

Designed by: John Szymanski   Group: iGEM08_Brown   (2008-07-01)
Revision as of 00:34, 20 October 2016 by Camy26 (Talk | contribs) (→‎Applications of BBa_K124000)

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Applications of BBa_K124000

Activation and Repression of CYC1 using scRNAs


CYC1_Characterization


Expression control of CYC1 promoter in yeasts. A histogram shows fluorescence intensities of GFP when it is produced under basal level, activation or repression. The basal level represents cells single-transfected with CYC1-GFP. For the activation, the strain was additionally transfected with dCas9, scCyc1_MS2 and the activation module MCP-VP64. For the repression, the scaffold RNA is scCyc1_PP7 and the transcriptional module is PCP-Mxi1. MS2 targets region c3 of the CYC1 promoter, while PP7 targets region c6. Then, with these basic elements, in principle all gates could be modularly assembled.

User Reviews

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