Composite
RBS + ButC

Part:BBa_K2052015:Design

Designed by: Burak Kızıl, Seniz Yuksel   Group: iGEM16_METU_HS_Ankara   (2016-09-24)
Revision as of 00:17, 20 October 2016 by Maercyl (Talk | contribs) (→‎Design Notes)


ButCoat with strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS and our protein coding region (ButCoaT) are digested from our whole construct with the enzymes Sall and HindIII.


In order to be expressed in E.coli BL21, the sequence was optimized with the use of codon bias rule and the unwanted restriction cut sides(SalI, HindIII) were removed with point mutation.

Source

............... Enter the source of this part. For example, does it come from some genomic sequence?

References