Reporter

Part:BBa_K2150028

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-13)
Revision as of 22:20, 19 October 2016 by Forevyoung (Talk | contribs)


pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT

In this part, T7 RNA polymerase(T7RNAP) is expressed from a tetracycline inducible promoter pTet. T7RNAP can activate the T7 promoter, thus producing the fusion protein in large quantity.


Usage and Biology

We design this part to further enhance the degrading capacity of our scavenger. The amount of tetX is reflected by the fluorescent intensity, and the degradation of tetracycline is indicated by the growth of the cell.

Characterization

Captain Scavenger.png


As shown in Fig.1, compariing to BBa_K2150015, this part proforms better in the presence of tetR, at all Tc concentration applied. Bacteria carrying this part grow better, and produce a larger amount of degrading-enzyme (represented by fluorescence intensity) in all than the Bacteria carrying BBa_K2150015.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4703


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Categories
Parameters
None