Part:BBa_K1918103

TRE-Kozak-EBFP2N(1-154):IntN

This part is consist of EBFP2N(1-154):IntN and the TRE promoter. EBFP2N(1-154):IntN is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2 and IntN. TRE is the Dox induced promoter. Driven by TRE promoter, the expression of EBFP2N(1-154):IntN could be induced by Dox.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 379
  Illegal XbaI site found at 1123
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 379
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 379
  Illegal BamHI site found at 362
  Illegal XhoI site found at 19
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 379
  Illegal XbaI site found at 1123
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 379
  Illegal XbaI site found at 1123
  
• 1000
  COMPATIBLE WITH RFC[1000]

In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.




Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.