Reporter

Part:BBa_K2150017

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 21:57, 19 October 2016 by Forevyoung (Talk | contribs)


pT7 TetX-GFP(fusion protein)

T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP BBa_K2150013) with double terminator.


Usage and Biology

This part is designed to produce a high level of the degrading enzyme in the presence of T7 RNA polymerase(see BBa_K21500028).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1905


[edit]
Categories
Parameters
None