Regulatory

Part:BBa_K1913024

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)
Revision as of 21:50, 19 October 2016 by Lisaro (Talk | contribs)


Synthetic ptet-FixK2 hybrid promoter with RBS

Synthetic ptet-FixK2 hybrid promoter is a promoter wich can not only be activated by light sensors YF1-FixJ composite but also inhibited by the TetR repressor. The sequence of the upper control element is from wild type FixK2 promoter on the genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. The core element region is replaced by the core element region of BBa_J23106. Additional tet operators are inserted into the core element region of promoter as second control elements which result in transcription repression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 118
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 27
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None