Regulatory

Part:BBa_K2117004

Designed by: Isabella Loft   Group: iGEM16_DTU-Denmark   (2016-10-14)
Revision as of 21:45, 19 October 2016 by CamillaKrogsgaard (Talk | contribs)


SCR1'-tRNA

This is a promoter optimized for expression of sgRNA in Yarrowia Lipolytica.

sgRNA is one of the components needed for a functional CRISPR-Cas9 system. The sgRNA guides the protopacer to the gene of interest inducing a double stranded break.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 29
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None