Regulatory

Part:BBa_K1913023

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)
Revision as of 21:44, 19 October 2016 by Lisaro (Talk | contribs)


Synthetic plac-FixK2 hybrid promoter with RBS

Synthetic plac-FixK2 hybrid promoter is a promoter which can not only be activated by light sensors YF1-FixJ but also inhibited by LacI repressor. The sequence of the upper control element is from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. The core element region is replaced by core element region of ompC promoter from another two-component system, EnvZ-OmpR in Escherichia coli. Additional lac operators on the both side of promoter as second control elements that result in transcription repression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None