Coding

Part:BBa_K1985014

Designed by: Rita Adriani   Group: iGEM16_Kent   (2016-10-14)
Revision as of 21:28, 19 October 2016 by R.adriani (Talk | contribs)

Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter

This part is an improved version of the fusion protein(BBa_K1739003) designed by the Kent 2015 iGEM team. It contains four segments, an arabinose inducible promoter designed by the Imperial 2014 iGEM team (Part:BBa_K1321333), the CsgA signal sequence, the first 61 aminoacids of the prion domain Sup35 and the electron transfer protein cytochrome 562. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures***. The addition of the arabinose inducible promoter allows for tighter control for the expression of amyloid fibres rather than a constitutive promoter as was previously used.


Validation

The improved plasmid was validated in three ways:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None