DNA

Part:BBa_K1896019:Design

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)
Revision as of 20:25, 19 October 2016 by Bvh (Talk | contribs)

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pXS-GG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 77
    Illegal BsaI.rc site found at 66


Design Notes

If Golden Gate assembly is used to clone fragments into this part, the resulting composite parts should be indistinguishable from those created by standard Registry assembly methods. The promoter used in this part may be too strong to be useable for proteins that cause toxicity at high concentrations (e.g. membrane proteins). BBa_K1896020 was created as an alternative with a weaker promoter.


Source

N/A

References