Generator

Part:BBa_K1896018

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)
Revision as of 19:47, 19 October 2016 by Bvh (Talk | contribs)


INP_RC-mSA2 generator

This part contains the INPRC-mSA2 fusion protein controlled by a weak to medium constitutive promoter and RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 203
    Illegal NgoMIV site found at 1715
    Illegal NgoMIV site found at 1859
    Illegal NgoMIV site found at 2123
    Illegal NgoMIV site found at 2267
    Illegal NgoMIV site found at 2315
    Illegal NgoMIV site found at 2435
    Illegal AgeI site found at 1379
    Illegal AgeI site found at 3254
    Illegal AgeI site found at 3314
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Green, R. L., Corotto, L. V., & Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from Pseudomonas syringae S203. Molecular and General Genetics MGG, 215(1), 165-172.
  2. Mann, J. K., Demonte, D., Dundas, C. M., & Park, S. (2016). Cell labeling and proximity dependent biotinylation with engineered monomeric streptavidin. Technology, 1-7.
[edit]
Categories
Parameters
None