Reporter

Part:BBa_K2150016

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 19:41, 19 October 2016 by Forevyoung (Talk | contribs)


pT7 GFP

T7 promoter and RBS and GFP, with double terminator. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.


Usage and Biology

We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed the T7 promoter is leaky on high-copy plasmid pSB1C3.

PT7+GFP.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690


[edit]
Categories
Parameters
None