![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014000
Designed by: Przemyslaw Nuc Group: iGEM16_UAM_Poznan (2016-10-11)
pBAD-E15'UTR->sfGFP
pBAD-E15'UTR->sfGFP construct is derived from pBAD (Arashort1, BBa_K1741000) Escherichia coli K-12 arabinose promoter, which we fused with E1_5’UTR (Fig. 1). E1_5’UTR contains an additional ribosome binding site from gene 10 of bacteriophage T7. The new promoter controls the expression of sfGFP with a His-tag at its N-end. The fluorescent protein sfGFP is a marker of gene expression and protein synthesis/accumulation. Protein expression from all compared arabinose responsive promoters was induced in rich media with 0.4% L-arabinose.
[[[Image:UAMpoznanaraall.jpg|800px|Link=![]() |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 236
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 53
Illegal SapI.rc site found at 365
[edit]
Categories
Parameters
//awards/part_collection/2016
None |