Part:BBa_K1907004

TSA1 promoter for S. cerevisiae

Introduction

TSA1 is a gene for thioredoxin peroxidase - a protein that acts as both a ribosome-associated and a free cytoplasmic antioxidant. It self-associates to form a high-molecular weight chaperone complex under oxidative stress. As Tsa1p is produced under oxidative stress, its promoter is activated in these conditions. (Saccharomyces genome database ID: S000004490)

This part contains a promoter region (260 bp) of TSA1 gene. The length of promoter region is chosen so that it contains identified Skn7 and Yap1 binding sites and doesn’t overlap with the next gene in the genome. (He et al., 2005; SGD ID:S000004490) Skn7 and Yap1 are the main transcription factors activated in oxidative stress and are thus responsible for TSA1 promoter activation. The gene sequence for the TSA1 promoter region is taken from strain S288C of Saccharomyces cerevisiae, obtained from the Saccharomyces Genome Database.

The functionality of this promoter has been tested in association with the Venus YFP reporter in S. cerevisiae strain SS328-leu, and proven to be functional when oxidative stress is induced by hydrogen peroxide. The TSA1 promoter has a relatively high base level of expression, but expression levels are further driven up when induced.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 453
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  Illegal NgoMIV site found at 613
  Illegal NgoMIV site found at 623
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 158
  

References

He, X.J. and Fassler, J.S., 2005. Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae. Molecular microbiology, 58(5), pp.1454-1467.