Reporter

Part:BBa_K1943004:Experience

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-08-29)
Revision as of 18:22, 19 October 2016 by John Tsq (Talk | contribs) (→‎Applications of BBa_K1943004)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1943004

After we constructed this part, we transformed it into BL21 to test the expression efficiency. Meanwhile, we did a comparison with another part only whose promoter+RBS combination differs.

Expression test of BBa_K1943004

We spread the plate for DH5α at 1:20am on 14th, Oct. The plate contains chloramphenicol. Following photo were taken every several hours after placing the plates into a 37℃ incubator. K1943004 is in the up-left corner in each photo.

At 12:24 (14th,Oct.) 

6 7@1224.jpeg

At 14:20 (14th,Oct.) 

6 7@1420.jpeg

At 18:30 (14th,Oct.) 

6 7@1830.jpeg

At 20:21 (14th,Oct.) 

6 7@2021.jpeg

At 22:40 (14th,Oct.) 

6 7@2240.jpeg

At 8:50 (15th,Oct.) 

6 7@850.jpeg

At 14:28 (15th,Oct.) 

6 7@1428.jpeg

User Reviews

UNIQb6f992ef2fe83993-partinfo-00000000-QINU UNIQb6f992ef2fe83993-partinfo-00000001-QINU