Generator

Part:BBa_K1979005

Designed by: MA Xinyi   Group: iGEM16_SDSZ_China   (2016-09-17)
Revision as of 17:02, 19 October 2016 by Xinyiloowhoo (Talk | contribs)


CBD-PBP5

This part codes for CBD (cellulose binding domain)- PBP5 protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 135
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1322
    Illegal BamHI site found at 168
    Illegal XhoI site found at 1530
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 636
    Illegal AgeI site found at 1238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 184




Figure2. M: Marker; 1-3: deposit of E.coli after centrifugation
The CBD-PBP5 protein (red box) is expressed, with a molecular weight of 53kDa. E.coli in channel 3 is the blank comparison.


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Categories
Parameters
None