Measurement

Part:BBa_I20245

Designed by: Jason Kelly, Karen Wong, Nishant Bhat   Group: Endy Lab   (2007-08-15)
Revision as of 15:15, 15 August 2007 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


A device used to characterize promoters throught measurement of GFP expression

Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. This design best simulates spacing in actual construction however, parts cannot be removed after testing.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 684


[edit]
Categories
Parameters
None