Part:BBa_K1959003
BKT coding sequence fused with Pea transit peptide
This part contains the coding sequence (CDS) of β-carotene ketolase (BKT, EC 1.14.11.B16) of algae (Chlamydomonas reinhardti), which catalyzes the conversion of zeaxanthin to astaxanthin. A Pea transit peptide of RUBISCO small subunit has been fused to BKT and the codon has been optimized for rice (Oryza sativa).
Usage and Biology
As a strong antioxidant, astaxanthin synthesis, especially, its biosynthesis attracts much interest of scientists. BKT symmetrically introduces two keto groups to two β-ionone rings of zeaxanthin to generate astaxanthin in algae (Figure.1).
In our reconstructed pathway, the CDS of BKY was codon-optimized for rice. In addition, a Pea transit peptide was fused to the BKY for proper sorting into the plastid.
Figure. 1 The reconstructed biosynthesis pathway of astaxanthin in the endosperm of aSTARice.
The dotted arrows indicate pathway is absent in rice endosperm. The solid arrows indicate the existence of carotenogenic reactions. The red arrows indicate the reactions catalyzed by four exogenous transgenic PSY, CrtI, BHYand BKT.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1085
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 478
Illegal SapI site found at 900
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