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Part:BBa_K2100010:Experience

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)
Revision as of 16:10, 19 October 2016 by Sarahcaso (Talk | contribs)


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Applications of BBa_K2100010

We characterized our hybrid promoter in both MCF-7 and tHESC cells. The following characterization experiment in tHESC cells tested how the hybrid promoter responds to a 1 uM induction of progesterone (MPA). The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.

T--MIT--hybridthesc.png

The results showed an 100 fold difference between the yellow fluorescent output of the uninduced MCF-7 cells and the ones induced with 10 nM E2.

The next characterization experiment tested how the hybrid promoter responds to a 10 nM induction of E2 (estradiol) in MCF-7 cells. The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.

T--MIT--hybridmcf7.png

The results showed a 12 fold difference between the yellow fluorescent output of the uninduced MCF-7 cells and the ones induced with 10 nM E2.


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