Coding

Part:BBa_K1959003

Designed by: Junyu Chen   Group: iGEM16_SCAU-China   (2016-10-04)
Revision as of 16:03, 19 October 2016 by Chenjunyu (Talk | contribs)


BKT coding sequence fused with Pea transit peptide

This part contains the coding sequence (CDS) of β-carotene ketolase (BKT, EC 1.14.11.B16) of algae (Chlamydomonas reinhardti), which catalyzes the conversion of zeaxanthin to astaxanthin. A Pea transit peptide of RUBISCO small subunit has been fused to BKT and the codon has been optimized for rice (Oryza sativa).


Usage and Biology

　As a strong antioxidant, astaxanthin synthesis, especially, its biosynthesis attracts much interest of scientists. BKT symmetrically introduces two keto groups to two β-ionone rings of zeaxanthin to generate astaxanthin in algae (Figure.1).
　In our reconstructed pathway, the CDS of BKY was codon-optimized for rice. In addition, a Pea transit peptide was fused to the BKY for proper sorting into the plastid.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 478
    Illegal SapI site found at 900


[edit]
Categories
Parameters
None