Intermediate

Part:BBa_K2036007

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 15:56, 19 October 2016 by Tianxiongxiao (Talk | contribs)


RBS-CII-RBS-CII, tandem expression

CII is a transcriptional activator of pRE, but it is normally degrade by Ftsh, and in this case,tandom expression maybe useful to activate pRE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Protein&promoter

--CII and pRE


CII (BBa_K2036000) functions as a transcriptional activator to direct promoter RE, so we constructed CII-TT-pRE-RBS-GFP-LVAssrAtag as test group and pRE-RBS-GFPLVAssrAtag as CK to see if CII efficiently activate pRE.


Fig1: According to the Flourescence measurement curve above, we can see clearly that GFP level increased over time and it showed significant difference from CK.


Fig2: We also did Fluorescence microscope detection after 30, 120 and 240 minutes induction. According to the figture below, we can tell qualitively that pRE leakage are at relative low level and CII can efficiently activate the promoter.
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Categories
Parameters
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