Part:BBa_K2170050:Design

Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2448
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 889
  

Keywords:

Abbreviations:

Design Notes

Related BioBrick:

• Other versions:BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin
  
• Related BioBricks:BBa_K2170051: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide

Cloning details:

• Designed in RFC10

Quality control measures:

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
• Tag: internal A3C5 Tag

Enzymatic activity:

• none

Cytotoxicity:

• none

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none

Source

Source:

• Preexisting BioBrick BBa_I739001; BBa_B0015
• Parts Synthesized by IDT
• Professor Dr. Arne Skerra (Chair biological chemistry of TUM)

Organism:
Genesequence derived from Escherichia coli

• Designed for the following Chassis: procaryotic cells

References

Literature references:

Database references: