Regulatory
cI lam

Part:BBa_R0051:Design

Designed by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross   Group: Antiquity   (2003-01-31)
Revision as of 14:42, 14 August 2007 by Scmohr (Talk | contribs) (→‎Source)

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promoter (lambda cI regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03)

Incompatible with host expressing cI repressor.



Source

"Synthetic oscillatory network of transcriptional regulators," Elowitz,M.B. and Leibler S., Nature 403, 335-38 (2000)

References