Generator

Part:BBa_K1976001:Design

Designed by: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Tim Maier   Group: iGEM16_TU_Darmstadt   (2016-10-14)
Revision as of 14:47, 19 October 2016 by Timmaier (Talk | contribs) (→‎Design Notes)

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λ-Integrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Promotor was added by restriction cloning. BBa_J61002_J23101 was used.

Source

Integrase origin: λ phage

Integrase and RBS were synthesized by iDT

References