Composite

Part:BBa_K1976000

Designed by: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Tim Maier   Group: iGEM16_TU_Darmstadt   (2016-10-13)
Revision as of 14:32, 19 October 2016 by Timmaier (Talk | contribs)


Integration plasmid

Usage and Biology

Green fluorescent protein with attachment-site-plasmid (attP) of phage λ. By genomic integration of this part into bacteria carrying the bacterial attachment site (attB), the metabolic burden on the microorganism can be quantified measuring the GFP concentration. The genomic integration is achieved by attP/attB recombination and catalysed by the λ-integrase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 315
    Illegal NheI site found at 338
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1014


[edit]
Categories
//classic/plasmid/measurement
Parameters
None