Coding

Part:BBa_K2172009

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)
Revision as of 13:10, 19 October 2016 by Xiaobowen (Talk | contribs)


Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

This part can be used to make other parts that can be purified, cleaved and detected for expression conveniently. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin protease cleavage site and a GFP detection unit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 747
    Illegal XhoI site found at 1485
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


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Categories
Parameters
None