Device

Part:BBa_K2170001:Design

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-11)
Revision as of 13:03, 19 October 2016 by LMU TUM Munich (Talk | contribs) (→‎Design Notes)


Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2432


Design Notes

Related BioBrick:
Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin
Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA

Cloning details:
Designed in RFC25

Quality control measures:
Test digestion using EcorRI & PstI Sequencing using VF2 Part was partly sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp Copynumber: high

Protein coding:
Protein: Secretory eukaryotic biotin binding receptor with single chain avidin [Nucleotide 633 to 3314]


Enzymatic activity: none

Cytotoxicity:
not known

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none

Intellectual property:

Corresponding part author/authors:

Source

Source:
Parts synthesized by IDT


Organism:
Genesequence derived from Escherichia coli


References

Literature references:

Database references: