Part:BBa_K1930006

sfGFP(Sp)

sfGFP(Sp) is a reporter gene originally optimized for Streptococcus pneumoniae.

It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs). [1].

Usage and Biology

E.coli appears green, when grown with this BioBrick.

Figure 1. sfGFP(Sp) in E.coli Top10.

When the sfGFP is integrated into the genome of B. subtilis 168 the colonies do not appear green by eye but under the microscope the GFP signal is well seen.

Figure 2. sfGFP(Sp) in B. subtilis 168.

Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]