Device

Part:BBa_K2170001:Design

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-11)
Revision as of 12:58, 19 October 2016 by LMU TUM Munich (Talk | contribs) (→‎Design Notes)


Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2432


Design Notes

Related BioBrick:
Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin
Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA

Cloning details:
Designed in RFC25 Mutation C889G to delete XbaI restriction site Truncation upstream/downstream compared to template, ?explanation?

Quality control measures:
Test digestion using EcorRI & PstI Sequencing using VF2 Part was partly sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Corresponding part author/authors:

Source

Source:
Parts synthesized by IDT


Organism:
Genesequence derived from Escherichia coli


References

Literature references:

Database references: