Generator

Part:BBa_K1934061:Experience

Designed by: Mathilde Gonthier   Group: iGEM16_INSA-Lyon   (2016-10-13)
Revision as of 11:55, 19 October 2016 by Suxiaohui (Talk | contribs)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1934061

This part has been transferred into E. coli NM522 for expression. After 6 hours at 37°C, IPTG was added to a final concentration of 1mM. The expression culture was grown overnight at 30°C. We then used instructions provided on the Ni-NTA columns kit from Qiagen to purify the protein.

User Reviews

UNIQd231d6e19caeb145-partinfo-00000000-QINU UNIQd231d6e19caeb145-partinfo-00000001-QINU