Device

Part:BBa_K1919200

Designed by: Haoyu Wang   Group: iGEM16_SCU-China   (2016-10-14)
Revision as of 11:40, 19 October 2016 by Steam (Talk | contribs)


J23100 promoter-Constitutive AarC Expression Device

This device can express AarC with His-tag. Promoter is BBa_J23100. His-tag and T7 terminator come from pET-23 vector.


Usage and Biology

Figure 1: Fermentation of E. coli KRXwith BBa_K863005 (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO2 at 50 %, OD600 measured every 30 minutes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 168
    Illegal AgeI site found at 264
    Illegal AgeI site found at 276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114
    Illegal BsaI.rc site found at 378


[edit]
Categories
Parameters
None