![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K1919214
Designed by: Haoyu Wang Group: iGEM16_SCU-China (2016-10-14)
J23114 promoter-Constitutive AarC Expression Device
This device can express AarC with His-tag. Promoter is BBa_J23114. His-tag and T7 terminator come from pET-23 vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1578
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 168
Illegal AgeI site found at 264
Illegal AgeI site found at 276 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 114
Illegal BsaI.rc site found at 378
![](/wiki/images/0/08/EH-0.png)
Figure 1: Fermentation of E. coli KRXwith BBa_K863005 (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO2 at 50 %, OD600 measured every 30 minutes.
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Categories
Parameters
None |