Reporter

Part:BBa_K2060001:Design

Designed by: Cardiff_Wales Team, Geraint Parry   Group: iGEM16_Cardiff_Wales   (2016-09-23)
Revision as of 11:05, 19 October 2016 by Gparry75 (Talk | contribs)

Design

The mKeima sequence was taken from GenBank and was synthesised as an [http://www.idtdna.com/pages/products/genes/gblocks-gene-fragments IDT gBlock] downstream of the well-characterised pBAD Arabinose inducible promotor and a RBS (tcacacaagaagg) together with the Biobrick Prefix and Suffix. This gBlock was 878bp in total.

We cloned the gBlock into pSB1C3 by restriction digestion with EcoRI and PstI.

We confirmed the entire construct by sanger sequencing