Device

Part:BBa_K1946004

Designed by: Ayşe Naz Ozantürk   Group: iGEM16_Bilkent-UNAMBG   (2016-10-19)
Revision as of 10:27, 19 October 2016 by Registry (Talk | contribs)

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Toluene/Xylene Sensor

This part responds to the presence of toluene, m-, p- or o-xylenes and produces a green fluorescent signal. The transcriptional regulatory protein XylR recognizes these organic molecules and activates pu promoter which in turn will lead to the synthesis of sfGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2248
    Illegal BamHI site found at 1751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168
    Illegal BsaI.rc site found at 1546
    Illegal SapI.rc site found at 2266


[edit]
Categories
//function/sensor
Parameters
device_typexylene sensor