Part:BBa_B0030:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_B0030

William and Mary iGEM 2016

We characterized the strengths of the Anderson RBS library.

User Reviews

UNIQ6bdb6c6d388d026b-partinfo-00000000-QINU

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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Aberdeen_Scotland 2009

The miniprep, single and double digests all worked. However we did not use this part for further cloning.

UNIQ6bdb6c6d388d026b-partinfo-00000003-QINU

UNIQ6bdb6c6d388d026b-partinfo-00000004-QINU

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UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.

See Experience page of BBa_B0034 for more details.

Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter:

RBS	eff_{p_Lux}	eff_{p_Tet}	eff_J23101	Declared efficiency
B0030	0.40	1.6814	2.45	0,6
B0031	0.01	ND	0.04	0,07
B0032	0.19	0.4193	0.40	0,3
B0034	1	1	1	1

Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI:

RBS	eff_mRFP	eff_AiiA	eff_LuxI	Declared efficiency
B0030	1.72	0.96	0.45	0,6
B0031	0.03	0.78	0.028	0,07
B0032	0.37	0.51	N.D.	0.3
B0034	1	1	1	1

UNIQ6bdb6c6d388d026b-partinfo-00000007-QINU