Project

Part:BBa_K1926001:Design

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 06:37, 19 October 2016 by CindyLiu (Talk | contribs) (→‎References)

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A cyclic promoter of Cyclin E from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 382
    Illegal NotI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 529
    Illegal XhoI site found at 1050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 273
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 491
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got this sequence from human genome through PCR using the following primers:

pCCNE-F: CGTGTTTACATTCCACCCGCGCCA

pCCNE-R: TGATGGGGCTGCTCCGGCCT

(Product: 986)

Details are all at the main page.

Source

The sequence was retrieved from Addgene.

References

1. Koff, A., et al., Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell, 1991. 66(6): p. 1217-1228.

2. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.

3. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.