Part:BBa_K1161003

Violacein C

Violacein C (Vio)C is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.

Part Contributions

Team Washington 2016 has codon optimized this part for use in S. cerevisiae as an improvement to its functionality in that organism. The new part, along with its further documentation, can be found at https://parts.igem.org/Part:BBa_K2165000 as part K2165000.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1312
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]