Composite

Part:BBa_K2040120:Design

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-12)
Revision as of 02:59, 19 October 2016 by Chilam Poon (Talk | contribs) (→‎Design Notes)

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KillerRed + TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 151
    Illegal BsaI.rc site found at 442


Design Notes

One illegal cutsite (EcoRI) were subsequently removed via site-directed mutagenesis.

Source

KillerRed was engineered from anm2CP.1 TtrpC originated from Aspergillus nidulans was isolated from pBARGPE1.

References