Device

Part:BBa_K2040121:Design

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-14)
Revision as of 02:57, 19 October 2016 by Chilam Poon (Talk | contribs) (→‎Source)


PgpdA + KillerRed + TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 880
    Illegal BsaI.rc site found at 1171


Design Notes

KillerRed can be replaced by KillerRed-NLS so that KillerRed can be localized in the nucleus.


Source

KillerRed was engineered from anm2CP.1[1]

PgpdA,TtrpC ,from Aspergillus nidulans, were isolated from vector pBARGPE1.

References

[1]Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A.
A Genetically Encoded Photosensitizer
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175