Part:BBa_K1998000

Mg-Chelatase Plasmid

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4753
  Illegal NotI site found at 2666
  Illegal NotI site found at 4213
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 439
  Illegal BglII site found at 1095
  Illegal BglII site found at 1290
  Illegal BglII site found at 3371
  Illegal BglII site found at 3898
  Illegal BglII site found at 5755
  Illegal BglII site found at 5863
  Illegal BamHI site found at 487
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1946
  Illegal NgoMIV site found at 2765
  Illegal NgoMIV site found at 3307
  Illegal NgoMIV site found at 7968
  Illegal AgeI site found at 6853
  Illegal AgeI site found at 6883
  Illegal AgeI site found at 7777
  Illegal AgeI site found at 7945
  Illegal AgeI site found at 9463
  Illegal AgeI site found at 9517
  Illegal AgeI site found at 9736
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 5553
  Illegal BsaI.rc site found at 5709
  Illegal BsaI.rc site found at 6330
  Illegal BsaI.rc site found at 9862
  Illegal SapI.rc site found at 7219
  Illegal SapI.rc site found at 9792
  

Overview

This composite part is made up of various genes that form a part of the chlorophyll biosynthesis pathway. ChlI1 and ChlI2 forms a Mg-chelatase complex which catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. ChID forms an ATP dependent complex with ChlI1 and ChlI2 before acting of protoporphyrin. GUN4 is a tetrapyrrole-binding protein which controls the production of Mg-protoporphyrin IX found only in aerobic photsynthetic organisms. It is thought to participate in plastid-to-nucleus signalling by regulating Mg-protoporphyrin IX synthesis. Finally, ChlH is a chloroplast precursor which plays a role in the regulation of light in chlorophyll biosynthesis.

CTH1 is a functional protein that is developed in the prescense of oxygen or copper. It catalyzes Mg-protoporphyrin IX monomethyl, which converts to divinyl protochlorophyllide in the presence of NADPH and O2.

This biobrick contains six genes from the chlorophyll biosynthetic pathway of Chlamydomonas reinhardtii. These genes are Magnesium chelatase subunit H (ChlH), Magnesium chelatase subunit I (Chli1/Chli2), Magnesium chelatase subunit D (ChlD), Genomes uncoupled 4 (GUN4) and Copper target 1 protein (Cth1). These genes are involved in converting protoporphyrin IX to divinyl protochlorophyllide in the presence of NADPH and O2.

Protein information

YCF54
mass: 17.08kDa
sequence: MAPAAASADKATAAEYYALVCNAEWFFMDPQNESVAEQLREKVRFFKEQN KERDFFIVPNPKWLDAKFPEQAKQVKRPCVALVSTDKMWITFMKLRLDRV LKIDLKSMPASEVLAAGEALPDFKPDGKWTAPYARYTPGWWNVFLPNH


ChlM
mass: 30.45kDa sequence: MASEIAQTADVGSLTFAVGGVGAVVGLGALLVATDHQKRRSEQMKSFDGD EKEAVKDYFNTAGFERWRKIYGETDEVNKVQLDIRTGHAQTVDKVLRWVD EEGSVQGITVADCGCGTGSLAIQLALRGAAVSASDISAAMASEAEQRYQQ AVAAGQGKAPKVAPKFEALDLESVKGKYDTVTCLDVMIHYPQDKVDAMIT HLAGLSDRRLIISFAPKTLSYSILKRIGELFPGPSKATRAYLHREEDVEAALK RAGFKVTKREMTATSFYFSRLLEAIRE