Coding

Part:BBa_K2065004:Experience

Designed by: Tom van Sonsbeek   Group: iGEM16_TU-Eindhoven   (2016-10-10)
Revision as of 00:15, 19 October 2016 by Tjvsonsbeek (Talk | contribs) (→‎iGEM TU Eindhoven 2016)

Applications of BBa_K2065000

iGEM TU Eindhoven 2016

iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This system was linked to CT52 (CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007") These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki "http://2016.igem.org/Team:TU-Eindhoven".

T--TU-Eindhoven--NanolUcreadoutbb.png
Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52