Composite

Part:BBa_K2149017:Design

Designed by: Aline Larissa Gonçalves   Group: iGEM16_USP-EEL-Brazil   (2016-10-18)
Revision as of 23:54, 18 October 2016 by AlineGoncalves (Talk | contribs) (→‎References)


Operon Lux C,E and D with an aldehyde decarbonylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2443
    Illegal NheI site found at 3827
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3215
    Illegal BamHI site found at 552
    Illegal BamHI site found at 2382
    Illegal BamHI site found at 3608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 466
    Illegal AgeI site found at 2185
    Illegal AgeI site found at 2400
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3649
    Illegal SapI.rc site found at 2020


Design Notes

This construction has a RBS before each gene (Lux C, Lux E and Lux D) and a UTR sequence before the gene that encodes an aldehyde decarbonylase.


Source

The sequences of operon Lux C, E and D was taken from Photorhabdus luminescens as describe by the work of Dr. Howard and collaborators called "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli". The gene of the aldehyde decarbonylase was taken from Synechococcus elongatus, a unicellular cyanobacterium and add after the genes of operon Lux.

HOWARD, T. P. et al. Synthesis of customized petroleum-replica fuel molecules by targeted modi fi cation of free fatty acid pools in Escherichia coli. PNAS, p. 1–6, 2013.