Coding

Part:BBa_K2114020

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-10)
Revision as of 22:46, 18 October 2016 by WladStr (Talk | contribs)


CgeA_G4S_HA_GST

C-terminal fusion of glutathione S-transferase to spore crust gene cgeA by a flexible GGGGS linker.


Usage and Biology

Figure 1: Schematic representation of the fusion protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1129
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526


[edit]
Categories
Parameters
None