Coding

Part:BBa_K1998013

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-13)
Revision as of 22:18, 18 October 2016 by SWinchester (Talk | contribs) (→‎Protein information)


MgPPIX to Chlorophyll a plasmid


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 912
    Illegal BglII site found at 2365
    Illegal BglII site found at 5647
    Illegal BglII site found at 6136
    Illegal BamHI site found at 316
    Illegal BamHI site found at 3128
    Illegal BamHI site found at 5416
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1904
    Illegal AgeI site found at 1955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 478
    Illegal BsaI.rc site found at 4018


Overview

This composite part makes up one of our operons in the chlorophyll biosynthesis pathway. It is composed of YCF54, ChlM, POR, DVR1, ChlP and ChlG. These genes are Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase (YCF54) and Magnesium-protoporphyrin O-methyltransferase (ChlM), Light-dependent protochlorophyllidereductase (POR), 3,8-divinyl, Geranylgeranyl reductase(ChlP), protochlorophyllidea 8-vinyl reductase (DVR1) and Chlorophyll synthetase (ChlG).
These genes are part of the chlorophyll biosynthetic pathway and are involved in converting Mg-protoporphyrin IX to chlorophyll

ChlorophyllBiosynthesisDiagram

Biology & Literature

Protein information

YCF54
Mass: 17.08kDa
Sequence: MAPAAASADKATAAEYYALVCNAEWFFMDPQNESVAEQLREKVRFFKEQN KERDFFIVPNPKWLDAKFPEQAKQVKRPCVALVSTDKMWITFMKLRLDRV LKIDLKSMPASEVLAAGEALPDFKPDGKWTAPYARYTPGWWNVFLPNH


ChlM
Mass: 30.45kDa Sequence: MASEIAQTADVGSLTFAVGGVGAVVGLGALLVATDHQKRRSEQMKSFDGD EKEAVKDYFNTAGFERWRKIYGETDEVNKVQLDIRTGHAQTVDKVLRWVD EEGSVQGITVADCGCGTGSLAIQLALRGAAVSASDISAAMASEAEQRYQQ AVAAGQGKAPKVAPKFEALDLESVKGKYDTVTCLDVMIHYPQDKVDAMIT HLAGLSDRRLIISFAPKTLSYSILKRIGELFPGPSKATRAYLHREEDVEAALK RAGFKVTKREMTATSFYFSRLLEAIRE

POR Mass: 41.87kDa Sequence: MVVCAATATA PSPSLADKFK PNAIARVPAT QQKQTAIITG ASSGLGLNAA KALAATGEWH VVMACRDFLK AEQAAKKVGM PAGSYSILHL DLSSLESVRQ FVQNFKASGR RLDALVCNAA VYLPTAKEPR FTADGFELSV GTNHLGHFLL TNLLLDDLKN APNKQPRCII VGSITGNTNT LAGNVPPKAN LGDLSGLAAG VPAANPMMDG QEFNGAKAYK DSKVACMMTV RQMHQRFHDA TGITFASLYP GCIAETGLFR EHVPLFKTLF PPFQKYITKG YVSEEEAGRR LAAVISDPKL NKSGAYWSWS STTGSFDNK

DVR1 Mass: 37kDa Sequence: MAMAASRQAV RVAAAVDADY RKREPKDVRV LVVGPTGYIG KFVVKELVSR GYNVVAFARE NAGIKGKMGR EDIVKEFHGA EVRFGSVLDP ASLRDVAFKD PVDVVVSCLA SRTGGKKDSW LIDYTATKNS LDVARASGAK HFVLLSAICV QKPLLEFQKA KLQFESDLQA AGDITYSIVR PTAFFKSIAG QIDIVKKGNP YVMFGDGNLA ACKPISEADL ASFIADCVTE QNKVNKVLPI GGPSKAFTAK QQADLLFNIT GLPPKYFPVP VALMDGMIGL FDSLAKLFPQ LEDSAEFARI GKYYATESML VYDEARGVYR KTKRLVTART RWKTSSLVQ

ChlP Mass: 47kDa Sequence: MVIGGGPSGA CAAETLAKGG VETFLLERKL DNCKPCGGAI PLCMVEEFDL PMEIIDRRVT KMKMISPSNR EVDVGKTLSE TEWIGMCRRE VFDDYLRNRA QKLGANIVNG LFMRSEQQSA EGPFTIHYNS YEDGSKMGKP ATLEVDMIIG ADGANSRIAK EIDAGEYDYA IAFQERIRIP DDKMKYYENL AEMYVGDDVS PDFYGWVFPK YDHVAVGTGT VVNKTAIKQY QQATRDRSKV KTEGGKIIRV EAHPIPEHPR PRRCKGRVAL VGDAAGYVTK CSGEGIYFAA KSGRMAAEAI VEGSANGTKM CGEDAIRVYL DKWDRKYWTT YKVLDILQKV FYRSNPAREA FVELCEDSYV QKMTFDSYLY KTVVPGNPLD DVKLLVRTVS SILRSNALRS VNSKSVNVSF GSKANEERVM AA

References

[1]

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Categories
Parameters
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