RNA

Part:BBa_K1941002:Design

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)
Revision as of 21:43, 18 October 2016 by Alixfailletaz (Talk | contribs) (→‎Design Notes)

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scTef1_2PP7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The overall structure of the scRNA was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting TEF1 from "CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes", L.A. Gilbert et al., Cell 2013. We also added a second PP7 hairpin, to asses if the repression was more efficient than with only one time the PP7 hairpin. To connect the two hairpins, we used an 18 bp linker also described in "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds".

Source

some source!

References